There were 12.8% acidic oligosaccharides in hydrolysate, eluted with the soluble xylan of DP> 8 together. Using the proper elution flow rate and injection volume at 800? mL/ h and 120? 酶解液中含有12.8%的酸性低聚糖,在洗脱时和木八以上的可溶性聚糖混合在一起。
The experimental results show that the GPC elution volume is a linear function of concentration. 结果表明:GPC淋洗体积(V(es))与浓度c呈线性函数关系。
The optimal technology contained the loading sample and the elution concentration and volume of alcohol. 优化工艺条件包括树脂载样量、稀醇洗脱浓度和体积,有效部位洗脱液醇浓度和体积等参数考察。
And the optimum elution conditions were as follows: elute volume 4 BV, elute concentration 70%, elute rate 2 BV/ h, pH value 8.0.The purity of the obtained limonoids was 37.26%. 最佳洗脱剂为70%乙醇溶液,洗脱液pH值为8.0,洗脱速率为2BV/h,洗脱体积为4BV。纯化得到的样品柠檬苦素类物质含量为37.26%。
Elution volume increases with the increase of concentration of the sam-ple. 浓度再增加,淋洗体积随之增加。